Plucked piezoelectric bimorphs for knee-joint energy harvesting: modelling and experimental validation

The modern drive towards mobility and wireless devices is motivating intensive research in energy harvesting technologies. To reduce the battery burden on people, we propose the adoption of a frequency up-conversion strategy for a new piezoelectric wearable energy harvester. Frequency up-conversion increases efficiency because the piezoelectric devices are permitted to vibrate at resonance even if the input excitation occurs at much lower frequency. Mechanical plucking-based frequency up-conversion is obtained by deflecting the piezoelectric bimorph via a plectrum, then rapidly releasing it so that it can vibrate unhindered; during the following oscillatory cycles, part of the mechanical energy is converted into electrical energy. In order to guide the design of such a harvester, we have modelled with finite element methods the response and power generation of a piezoelectric bimorph while it is plucked. The model permits the analysis of the effects of the speed of deflection as well as the prediction of the energy produced and its dependence on the electrical load. An experimental rig has been set up to observe the response of the bimorph in the harvester. A PZT-5H bimorph was used for the experiments. Measurements of tip velocity, voltage output and energy dissipated across a resistor are reported. Comparisons of the experimental results with the model predictions are very successful and prove the validity of the model

Pizzicato excitation for wearable energy harvesters

A new technique based on the plucking of flexible piezoelectric material can be used to boost energy harvested to power portable electronic devices

Low-profile and wearable energy harvester based on plucked piezoelectric cantilevers

The Pizzicato Energy Harvester (EH) introduced the technique of frequency up-conversion to piezoelectric EHs wearable on the lateral side of the knee-joint. The operation principle is to pluck the piezoelectric bimorphs with plectra so that they produce electrical energy during the ensuing mechanical vibrations. The device presented in this work is, in some ways, an evolution of the earlier Pizzicato: it is a significantly more compact and lighter device; the central hub holds 16 piezoelectric bimorphs shaped as trapezoids, which permits a sleek design and potentially increased energy output for the same bimorph area. Plectra were formed by Photochemical Machining of a 100-μm-thick steel sheet. To avoid the risk of short-circuiting, the plectra were electrically passivated by sputtering a 100 nm layer of ZrO2. Bench tests with the steel plectra showed a very large energy generation. Polyimide plectra were also manufactured with a cutting plotter from a 125μm-thick film. Besides bench tests, a volunteer wore the device while walking on flat ground or climbing stairs, with a measured energy output of approximately 0.8 mJ per step. Whereas most of the tests were performed by the traditional method of discharging the rectified output from the EH onto a resistive load, tests were performed also with a circuit offering a stabilised 3.3 V supply. The circuit produced a stable 0.1 mA supply during running gait with kapton plectra

Performance testing of a low power consumption wireless sensor communication system integrated with an energy harvesting power source

This paper presents the performance testing results of a wireless sensor communication system with low power consumption integrated with a vibration energy harvesting power source. The experiments focus on the system’s capability to perform continuous monitoring and to wirelessly transmit the data acquired from the sensors to a user base station, completely battery-free. Energy harvesting technologies together with system design optimisation for power consumption minimisation ensure the system’s energy autonomous capability demonstrated in this paper by presenting the promising testing results achieved following its integration with Structural Health Monitoring (SHM) and Body Area Network (BAN) applications

Synchronous Lung Cancers: When Same Histological Types Feature Different Molecular Profiles and Response Phenotypes

We discuss the case of synchronous bilateral lung cancers which feature the same histological phenotype and a different EGFR mutational profile. Both histological and molecular characterizations were performed on specimens derived thorough CT-guided fine needle aspiration. A first-line chemotherapy was unsuccessful. Subsequent objective response to the EGFR inhibitor Erlotinib was clearly coherent with the sequencing data and the mutated nodule was effectively reduced (> 50%) after therapy, while the lesion assessed as EGFR wild type featured a slight response. This report has two relevant implications. It points out that in case of multiple malignant lesions at time of diagnosis, molecular profiling should be as extensive as possible and it might contribute to clarify the association between the lesions found. Besides the molecular analysis on cytology specimens could identify an accurate and safe diagnostic approach for clinical use

Factors Influencing Oxidative Imbalance in Pulmonary Fibrosis: An Immunohistochemical Study

Background. Idiopathic Pulmonary Fibrosis (IPF) is a fatal lung disease of unknown etiology characterized by interstitial fibrosis determining irreversible distortion of pulmonary architecture. Reactive oxygen species (ROS) and markers of oxidative stress play a pivotal role in human IPF pathology, possibly through induction of epithelial-mesenchymal transition (EMT). Methods. We investigated by immunohistochemistry, in UIP and COP tissue samples, the expression of most relevant markers of the molecular interplay involving RAGE, oxidant/antioxidant balance regulation, tissue nitrosylation, and mediators of EMT. Results. In both UIP and COP, the degree of RAGE expression was similarly high, while SODs and i-NOS, diffusely present in COP endoalveolar plugs, were almost absent in UIP fibroblast foci. A lower degree of tissue nitrosilation was observed in UIP than in COP. Conclusions. Fibroblast lesions of UIP and of COP share a similar degree of activation of RAGE, while antioxidant enzyme expression markedly reduced in UIP

Targeting EGFR in non-small-cell lung cancer: Lessons, experiences, strategies

SummaryCancer is a genetic disease and this concept is now widely exploited by both scientists and clinicians to design new targeted molecules. Indeed many data have already allowed us to ameliorate not only our knowledge about cancer onset, but also about patients treatment. Correlation between mutations in cancer alleles and drug response is a key point to identify drugs that match the genetic profile of each individual tumors. On the other hand, experience derived from inhibition of tyrosine kinase receptors has pointed out that targeted treatment is really successful only in a small subset of tumors. The latter are eventually addicted to those genetic alterations which are responsible for receptors activation and for the continued expression of their signalling. Overall these observations provide a strong rationale for a molecular-based diagnosis and patients selection for targeted therapies.This review analyses the current state of the art of molecularly-tailored pharmacological approach to lung cancer, one of the biggest killers among human solid tumors. Main relevance is addressed to genetic lesions activating the EGFR pathway transducers, focusing on their role as markers of targeted drug response

Successful whole lung lavage in pulmonary alveolar proteinosis secondary to lysinuric protein intolerance: a case report

BACKGROUND: Pulmonary alveolar proteinosis (PAP) is a rare disease characterised by accumulation of lipoproteinaceous material within alveoli, occurring in three clinically distinct forms: congenital, acquired and secondary. Among the latter, lysinuric protein intolerance (LPI) is a rare genetic disorder caused by defective transport of cationic amino acids. Whole Lung Lavage (WLL) is currently the gold standard therapy for severe cases of PAP. CASE PRESENTATION: We describe the case of an Italian boy affected by LPI who, by the age of 10, developed digital clubbing and, by the age of 16, a mild restrictive functional impairment associated with a high-resolution computed tomography (HRCT) pattern consistent with pulmonary alveolar proteinosis. After careful assessment, he underwent WLL. CONCLUSION: Two years after WLL, the patient has no clinical, radiological or functional evidence of pulmonary disease recurrence, thus suggesting that WLL may be helpful in the treatment of PAP secondary to LPI

Antitarget, Anti-SARS-CoV-2 Leads, Drugs, and the Drug Discovery-Genetics Alliance Perspective

: The most advanced antiviral molecules addressing major SARS-CoV-2 targets (Main protease, Spike protein, and RNA polymerase), compared with proteins of other human pathogenic coronaviruses, may have a short-lasting clinical efficacy. Accumulating knowledge on the mechanisms underlying the target structural basis, its mutational progression, and the related biological significance to virus replication allows envisaging the development of better-targeted therapies in the context of COVID-19 epidemic and future coronavirus outbreaks. The identification of evolutionary patterns based solely on sequence information analysis for those targets can provide meaningful insights into the molecular basis of host-pathogen interactions and adaptation, leading to drug resistance phenomena. Herein, we will explore how the study of observed and predicted mutations may offer valuable suggestions for the application of the so-called "synthetic lethal" strategy to SARS-CoV-2 Main protease and Spike protein. The synergy between genetics evidence and drug discovery may prioritize the development of novel long-lasting antiviral agents